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FCRC also has site license for De Novo Software FCS Express 7 for researchers to download and install on your personal computers for both MAC and PC OS. įCRC has three workstations where the FCS Express software is installed: Analysis PC3, Aurora-Client1 and Aurora-Client2, which can be used to analysis FCS files exported from any flow cytometry instrument. The help of Software Troubleshooting can be requested through PPMS system by filling up the FCRC-PPMS Request Form F4.įor more details about Flowjo please refer to the FlowJo website. FCRC also has one FlowJo dongle, which researchers could ask from FCRC staff and use on your personal computer for two hours or overnight (free of charge) after registration. The installed software on workstations is listed on FCRC Instruments and Analysis Workstations.įCRC has three workstations where the FlowJo software is installed: Analysis PC2, Aurora-Client1 and Aurora-Client2, which can be used to analysis FCS files exported from any flow cytometry instrument.

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All FCRC Analysis Workstations are available for booking via the PPMS system for usage free of charge, 24/7.

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There are five “Analysis PC” workstations and two “Aurora-Client” workstations with Windows 7 OS or Windows 10 OS, with multiple Flow Cytometry analysis software packages installed. We are also making changes to equipment in all of our location.The Flow Cytometry Resource Center (FCRC) provides the Rockefeller University researchers with multiple analysis workstations dedicated for the analysis of Flow Cytometry data. Beginning in mid June, the UFCR will be occupying 1200 sq. This same process may be performed on a cell sorting cytometer which additionally allows for the capture of subsets of the cells or particles to be re-used by the customer in a subsequent procedure.Įxciting changes are happening for UFCR with the opening of the Cancer-Cardio Research Building (CCRB), funding and key internal agreements. The result is an electronic file containing the measured information, which may be represented in a graphical format using specific software to interpret the file. Information about the size and granularity of a cell may be recorded, as well. The fluorescent signal is detected and amplified, then translated into an electronic signal, which is output to a computer workstation. The fluorescent probes are excited by the laser and then emit light energy.

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The cells then pass by a series of lasers, each of which emit a specific wavelength of light. Cells or particles tagged with fluorescent molecules enter the cytometer via a fluid stream. Please let the UFCR staff know if you have any questions.įlow cytometry uses fluorescent probes to identify and characterize cells or particles. We will let everyone know when we can revert back to our normal procedures. We aim to keep everyone healthy, so we can stay productive. Wash your hands with soap and water, cover your mouth when coughing or sneezing, and do not come to school or work when you do not feel well.īy following these guidelines we hope to minimize the chances of transmission of coronavirus or any other seasonal illness between our users and staff.

We encourage you to continue to follow the Minnesota Department of Health (MDH) guidance by taking the most basic measures to protect yourself-and all of us. Reminder: No food or drinks in cytometer rooms.Wipe instruments and work surfaces with sanitizing wipes or 70-80% ethanol before and after use.Wear gloves that have NOT been worn for sample or slide preparation when handling the instrument.Please send Sunrise plan AND letter of approval to Wash hands with soap and water or hand sanitizer before and after (washing stations available in each facility). Approval documentation for your lab MUST be on file with the UFCR for requests to be honored. Please use this link to request a reservation on our cell analyzer instrumentation.Many issues can and should be solved electronically to avoid unnecessary exposure to infectious agents. Contact us by email or telephone to set up an appointment. Do not show up at our offices unannounced.If you have a fever and/or are coughing/sneezing, please stay at home. We will gladly cancel any reservation at no charge due to illness.At the University Flow Cytometry Resource, dozens of people each week touch common work surfaces repeatedly (knobs, buttons, mouse, keyboard, etc.), so we have established a protocol with specific steps to minimize chances of viral transmission through this route: One established route of transmission for this virus is via contaminated surfaces.